Center of Excellence of the European Union
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About us
Research groups
Laszlo Buday
Karoly Liliom
Judit Ovadi
Laszlo Patthy
Istvan Simon
Gergely Szakacs
David Szuts
Peter Tompa
Gabor Tusnady
Andras Varadi
Beata Vertessy
Peter Zavodszky
Members
Publications
Selected publications
Articles
1.
Gál P
,
Dobó J
,
Beinrohr L
, Pál G and
Závodszky P
(2013) [22990693]
Inhibition of the serine proteases of the complement system.
Adv Exp Med Biol
734
, 23-40
2.
Gál P
,
Dobó J
,
Beinrohr L
, Pál G and
Závodszky P
(2013) [23402017]
Inhibition of the serine proteases of the complement system.
Adv Exp Med Biol
735
, 23-40
3.
Megyeri M
, Harmat V,
Major B
, Végh Á,
Balczer J
, Héja D,
Szilágyi K
, Datz D, Pál G,
Závodszky P
,
Gál P
and
Dobó J
(2013) [23386610]
Quantitative characterization of the activation steps of mannan-binding lectin (MBL)-associated serine proteases (MASPs) points to the central role of MASP-1 in the initiation of the complement lectin pathway.
J Biol Chem
288
, 8922-34
4.
Paréj K
,
Dobó J
,
Závodszky P
and
Gál P
(2013) [23399388]
The control of the complement lectin pathway activation revisited: Both C1-inhibitor and antithrombin are likely physiological inhibitors, while α(2)-macroglobulin is not.
Mol Immunol
54
, 415-422
5.
Csuka D, Munthe-Fog L, Skjoedt MO,
Kocsis A
, Zotter Z,
Gál P
, Varga L, Farkas H, Füst G and Garred P (2013) [23318225]
The role of ficolins and MASPs in hereditary angioedema due to C1-inhibitor deficiency.
Mol Immunol
54
, 271-277
6.
Hess K, Ajjan R, Phoenix F,
Dobó J
,
Gál P
and Schroeder V (2012) [22536427]
Effects of MASP-1 of the Complement System on Activation of Coagulation Factors and Plasma Clot Formation.
PLoS ONE
7
, e35690
7.
Thiel S, Jensen L, Degn SE, Nielsen HJ,
Gál P
,
Dobó J
and Jensenius JC (2012) [22670777]
Mannan-binding lectin (MBL)-associated serine protease-1 (MASP-1), a serine protease associated with humoral pattern-recognition molecules: normal and acute-phase levels in serum and stoichiometry of lectin pathway components.
Clin Exp Immunol
169
, 38-48
8.
Héja D, Harmat V, Fodor K, Wilmanns M,
Dobó J
, Kékesi KA,
Závodszky P
,
Gál P
and Pál G (2012) [22511776]
Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2.
J Biol Chem
287
, 20290-300
9.
Héja D,
Kocsis A
,
Dobó J
, Szilágyi K, Szász R,
Závodszky P
, Pál G and
Gál P
(2012) [22691502]
Revised mechanism of complement lectin-pathway activation revealing the role of serine protease MASP-1 as the exclusive activator of MASP-2.
P Natl Acad Sci Usa
109
, 10498-503
10.
Kidmose RT, Laursen NS,
Dobó J
, Kjaer TR, Sirotkina S, Yatime L, Sottrup-Jensen L, Thiel S,
Gál P
and Andersen GR (2012) [22949645]
Structural basis for activation of the complement system by component C4 cleavage.
P Natl Acad Sci Usa
109
, 15425-30
11.
Dobó J
,
Major B
, Kékesi KA, Szabó I,
Megyeri M
, Hajela K, Juhász G,
Závodszky P
and
Gál P
(2011) [21625439]
Cleavage of Kininogen and Subsequent Bradykinin Release by the Complement Component: Mannose-Binding Lectin-Associated Serine Protease (MASP)-1.
PLoS ONE
6
, e20036
12.
Kocsis J, Mészáros T, Madaras B,
Tóth EK
,
Kamondi S
,
Gál P
, Varga L, Prohászka Z and Füst G (2011) [20730518]
High levels of acute phase proteins and soluble 70 kDa heat shock proteins are independent and additive risk factors for mortality in colorectal cancer.
Cell Stress Chaperon
16
, 49-55
13.
Beinrohr L
, Murray-Rust TA, Dyksterhuis L,
Závodszky P
,
Gál P
, Pike RN and Wijeyewickrema LC (2011) [21683249]
Serpins and the complement system.
Method Enzymol
499
, 55-75
14.
Gulla KC, Gupta K, Krarup A,
Gal P
, Schwaeble WJ, Sim RB, OConnor CD and Hajela K (2010) [20002787]
Activation of mannan-binding lectin-associated serine proteases leads to generation of a fibrin clot.
Immunology
129
, 482-95
15.
Dobó J
,
Varga J
,
Sajó R
,
Végh BM
,
Gál P
,
Závodszky P
and
Vonderviszt F
(2010) [20008166]
Application of a short, disordered N-terminal flagellin segment, a fully functional flagellar type III export signal, to expression of secreted proteins.
Appl Environ Microb
76
, 891-9
16.
Degn SE, Jensen L,
Gál P
,
Dobó J
, Holmvad SH, Jensenius JC and Thiel S (2010) [20673767]
Biological variations of MASP-3 and MAp44, two splice products of the MASP1 gene involved in regulation of the complement system.
J Immunol Methods
361
, 37-50
17.
Major B
, Kardos J, Kékesi KA,
Lorincz Z
,
Závodszky P
and
Gál P
(2010) [20178990]
Calcium-dependent conformational flexibility of a CUB domain controls activation of the complement serine protease C1r.
J Biol Chem
285
, 11863-9
18.
Láng A,
Major B
,
Szilágyi K
, Gáspári Z,
Gál P
,
Závodszky P
and Perczel A (2010) [20970424]
Interaction between separated consecutive complement control modules of human C1r: Implications for dimerization of the full-length protease.
FEBS Lett
584
, 4565-4569
19.
Láng A,
Szilágyi K
,
Major B
,
Gál P
,
Závodszky P
and Perczel A (2010) [20796027]
Intermodule cooperativity in the structure and dynamics of consecutive complement control modules in human C1r: structural biology.
FEBS J
277
, 3986-98
20.
Kocsis A
, Kékesi KA, Szász R,
Végh BM
,
Balczer J
,
Dobó J
,
Závodszky P
,
Gál P
and Pál G (2010) [20817870]
Selective Inhibition of the Lectin Pathway of Complement with Phage Display Selected Peptides against Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 and -2: Significant Contribution of MASP-1 to Lectin Pathway Activation.
J Immunol
185
, 4169-78
21.
Than NG, Romero R, Goodman M, Weckle A, Xing J, Dong Z, Xu Y, Tarquini F,
Szilagyi A
,
Gal P
, Hou Z, Tarca AL, Kim CJ, Kim JS, Haidarian S, Uddin M, Bohn H, Benirschke K, Santolaya-Forgas J, Grossman LI, Erez O, Hassan SS,
Zavodszky P
, Papp Z and Wildman DE (2009) [19497882]
A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death.
P Natl Acad Sci Usa
106
, 9731-6
22.
Megyeri M
, Makó V,
Beinrohr L
, Doleschall Z, Prohászka Z, Cervenak L,
Závodszky P
and
Gál P
(2009) [19667088]
Complement protease MASP-1 activates human endothelial cells: PAR4 activation is a link between complement and endothelial function.
J Immunol
183
, 3409-16
23.
Gál P
,
Dobó J
,
Závodszky P
and Sim RB (2009) [19477526]
Early complement proteases: C1r, C1s and MASPs. A structural insight into activation and functions.
Mol Immunol
46
, 2745-52
24.
Dobó J
, Harmat V,
Beinrohr L
,
Sebestyén E
,
Závodszky P
and
Gál P
(2009) [19564340]
MASP-1, a Promiscuous Complement Protease: Structure of Its Catalytic Region Reveals the Basis of Its Broad Specificity.
J Immunol
183
, 1207-1214
25.
Hajdú I
,
Bőthe C
,
Szilágyi A
,
Kardos J
,
Gál P
and
Závodszky P
(2008) [18449535]
Adjustment of conformational flexibility of glyceraldehyde-3-phosphate dehydrogenase as a means of thermal adaptation and allosteric regulation.
Eur Biophys J Biophy
37
, 1139-44
26.
Beinrohr L
,
Dobó J
,
Závodszky P
and
Gál P
(2008) [18977695]
C1, MBL-MASPs and C1-inhibitor: novel approaches for targeting complement-mediated inflammation.
Trends Mol Med
14
, 511-21
27.
Varga L, Széplaki G, Laki J,
Kocsis A
, Kristóf K,
Gál P
, Bajtay Z, Wieslander J, Daha MR, Garred P, Madsen HO, Füst G and Farkas H (2008) [18460017]
Depressed activation of the lectin pathway of complement in hereditary angioedema.
Clin Exp Immunol
153
, 68-74
28.
Dobó J
, Harmat V, Sebestyén E,
Beinrohr L
,
Závodszky P
and
Gál P
(2008) [18765903]
Purification, crystallization and preliminary X-ray analysis of human mannose-binding lectin-associated serine protease-1 (MASP-1) catalytic region.
Acta Crystallogr F
64
, 781-4
29.
Kardos J
, Harmat V, Palló A,
Barabás O
,
Szilágyi K
, Gráf L, Náray-Szabó G, Goto Y,
Závodszky P
and
Gál P
(2008) [17996945]
Revisiting the mechanism of the autoactivation of the complement protease C1r in the C1 complex: structure of the active catalytic region of C1r.
Mol Immunol
45
, 1752-60
30.
Krarup A, Gulla KC,
Gál P
, Hajela K and Sim RB (2008) [18456010]
The action of MBL-associated serine protease 1 (MASP1) on factor XIII and fibrinogen.
Bba-proteins Proteom
1784
, 1294-300
31.
Beinrohr L
, Harmat V,
Dobó J
,
Lörincz Z
,
Gál P
and
Závodszky P
(2007) [17488724]
C1 inhibitor serpin domain structure reveals the likely mechanism of heparin potentiation and conformational disease.
J Biol Chem
282
, 21100-9
32.
Széplaki G, Varga L, Laki J, Dósa E, Rugonfalvi-Kiss S, Madsen HO, Prohászka Z,
Kocsis A
,
Gál P
, Szabó A, Acsády G, Karádi I, Selmeci L, Garred P, Füst G and Entz L (2007) [17916775]
Low c1-inhibitor levels predict early restenosis after eversion carotid endarterectomy.
Arterioscl Throm Vas
27
, 2756-62
33.
Gál P
,
Barna L
,
Kocsis A
and
Závodszky P
(2007) [17544812]
Serine proteases of the classical and lectin pathways: similarities and differences.
Immunobiology
212
, 267-77
34.
Krarup A, Wallis R, Presanis JS,
Gál P
and Sim RB (2007) [17637839]
Simultaneous activation of complement and coagulation by MBL-associated serine protease 2.
PLoS ONE
2
, e623
35.
Gál P
,
Végh B
,
Závodszky P
and
Vonderviszt F
(2006) [16900123]
Export signals.
Nat Biotechnol
24
, 900-1
36.
Végh BM
,
Gál P
,
Dobó J
,
Závodszky P
and
Vonderviszt F
(2006) [16674914]
Localization of the flagellum-specific secretion signal in Salmonella flagellin.
Biochem Bioph Res Co
345
, 93-8
37.
Gál P
, Harmat V,
Kocsis A
,
Bián T
,
Barna L
,
Ambrus G
,
Végh B
,
Balczer J
, Sim RB, Náray-Szabó G and
Závodszky P
(2005) [16040602]
A true autoactivating enzyme. Structural insight into mannose-binding lectin-associated serine protease-2 activations.
J Biol Chem
280
, 33435-44
38.
Presanis JS, Hajela K, Ambrus G,
Gál P
and Sim RB (2004) [14725788]
Differential substrate and inhibitor profiles for human MASP-1 and MASP-2.
Mol Immunol
40
, 921-9
39.
Harmat V,
Gál P
,
Kardos J
,
Szilágyi K
,
Ambrus G
,
Végh B
, Náray-Szabó G and
Závodszky P
(2004) [15364579]
The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions.
J Mol Biol
342
, 1533-46
40.
Sárvári M
, Vágó I, Wéber CS, Nagy J,
Gál P
, Mák M, Kósa JP,
Závodszky P
and Pázmány T (2003) [12667643]
Inhibition of C1q-beta-amyloid binding protects hippocampal cells against complement mediated toxicity.
J Neuroimmunol
137
, 12-8
41.
Ambrus G
,
Gál P
, Kojima M,
Szilágyi K
,
Balczer J
, Antal J, Gráf L, Laich A, Moffatt BE, Schwaeble W, Sim RB and
Závodszky P
(2003) [12538697]
Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments.
J Immunol
170
, 1374-82
42.
Gál P
,
Ambrus G
and
Závodszky P
(2002) [12396001]
C1s, the protease messenger of C1. Structure, function and physiological significance.
Immunobiology
205
, 383-94
43.
Hajela K, Kojima M, Ambrus G, Wong KH, Moffatt BE, Ferluga J, Hajela S,
Gál P
and Sim RB (2002) [12396008]
The biological functions of MBL-associated serine proteases (MASPs).
Immunobiology
205
, 467-75
44.
Lacroix M, Ebel C,
Kardos J
,
Dobó J
,
Gál P
,
Závodszky P
, Arlaud GJ and Thielens NM (2001) [11445589]
Assembly and enzymatic properties of the catalytic domain of human complement protease C1r.
J Biol Chem
276
, 36233-40
45.
Gal P
and Ambrus G (2001) [-100002667]
Structure and function of complement activating enzyme complexes: C1 and Mbl-masps.
Curr Protein Pept Sc
2
, 43-59
46.
Kardos J
,
Gál P
, Szilágyi L, Thielens NM,
Szilágyi K
,
Lőrincz Z
,
Kulcsár P
, Gráf L, Arlaud GJ and
Závodszky P
(2001) [11673533]
The role of the individual domains in the structure and function of the catalytic region of a modular serine protease, C1r.
J Immunol
167
, 5202-8
47.
Németh A
,
Svingor A
,
Pócsik M
,
Dobó J
,
Magyar C
,
Szilágyi A
,
Gál P
and
Závodszky P
(2000) [10683439]
Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase.
FEBS Lett
468
, 48-52
48.
Lörincz Z
,
Gál P
,
Dobó J
,
Cseh S
,
Szilágyi K
,
Ambrus G
and
Závodszky P
(2000) [10925288]
The cleavage of two C1s subunits by a single active C1r reveals substantial flexibility of the C1s-C1r-C1r-C1s tetramer in the C1 complex.
J Immunol
165
, 2048-51
49.
Dobó J
,
Gál P
,
Szilágyi K
,
Cseh S
,
Lörincz Z
, Schumaker VN and
Závodszky P
(1999) [9916740]
One active C1r subunit is sufficient for the activity of the complement C1 complex: stabilization of C1r in the zymogen form by point mutations.
J Immunol
162
, 1108-12
50.
Gál P
and
Závodszky P
(1998) [9777415]
Structure and function of the serine-protease subcomponents of C1: protein engineering studies.
Immunobiology
199
, 317-26
51.
Cseh S
,
Gál P
,
Sárvári M
,
Dobó J
,
Lőrincz Z
, Schumaker VN and
Závodszky P
(1996) [8676886]
Functional effects of domain deletions in a multidomain serine protease, C1r.
Mol Immunol
33
, 351-9
52.
Gál P
,
Cseh S
, Schumaker VN and
Závodszky P
(1994) [7866721]
The structure and function of the first component of complement: genetic engineering approach (a review).
Acta Microbiol Imm H
41
, 361-80
53.
Kovács A,
Gál P
and
Závodszky P
(1993) [8278582]
A simple method to assess in vivo repair of ultraviolet radiation-induced lesions of specific DNA sequences of restriction sites.
Radiat Res
136
, 397-403
54.
Závodszky P
,
Gál P
,
Cseh S
and Schumaker VN (1993) [8172556]
Protein engineering studies on C1r and C1s.
Behring Inst Mitt.
93
, 103-14
55.
Luo C, Thielens NM, Gagnon J,
Gal P
,
Sarvari M
, Tseng Y, Tosi M,
Zavodszky P
, Arlaud GJ and Schumaker VN (1992) [1533159]
Recombinant human complement subcomponent C1s lacking beta-hydroxyasparagine, sialic acid, and one of its two carbohydrate chains still reassembles with C1q and C1r to form a functional C1 complex.
Biochemistry-us
31
, 4254-62
56.
Sárvári M
, Csikós G, Sass M,
Gál P
, Schumaker VN and
Závodszky P
(1990) [2182025]
Ecdysteroids increase the yield of recombinant protein produced in baculovirus insect cell expression system.
Biochem Bioph Res Co
167
, 1154-61
57.
Gál P
,
Sárvári M
,
Szilágyi K
,
Závodszky P
and Schumaker VN (1989) [2557186]
Expression of hemolytically active human complement component C1r proenzyme in insect cells using a baculovirus vector.
notSet
6
, 433-41
58.
Vonderviszt F
, Lakatos S,
Gál P
,
Sárvári M
and
Závodszky P
(1987) [3675596]
A molten globule-like unfolding intermediate of a four domain protein, the Fc fragment of the IgG molecule.
Biochem Bioph Res Co
148
, 92-8
Books or book chapters
1.
Gal P
, Ambrus G,
Lorincz Z
and
Zavodszky P
(2004)
The initiation complexes of the classical and lectin patways.
The complement system. Novel roles in health and disease.
, 19-43